Proper selection, collection, and delivery of clinical specimens
when amassing scientific
specimens for microbiology, these widespread precautions must be taken:
The specimen ought to
be nicely decided on. That is, it must be the precise sort of specimen for the
analysis of the suspected infectious ailment.
The specimen needs to
be well and thoroughly accrued. On every occasion viable, specimens must be
accrued in a manner with the intention to get rid of or decrease contamination
of the specimen with indigenous microflora.
The cloth should be
accrued from a website where the suspected pathogen is most in all likelihood to
be located and where the least contamination is in all likelihood to occur.
Whenever feasible,
specimens must be obtained earlier than antimicrobial remedy has begun. If this
isn't feasible, the laboratory should be informed as to which antimicrobial
agent(s) the patient is receiving.
The acute degree of the
disorder (whilst the affected person is experiencing the signs and symptoms of
the ailment) is the maximum suitable time to collect maximum specimens. A few
viruses, however, are extra easily isolated for the duration of the prodromal
or onset level of the disease.
Specimen collection has
to be achieved with care and tact to keep away from harming the affected
person, inflicting discomfort, or inflicting undue embarrassment. If the
affected person is to gather the specimen, together with sputum or urine, the
affected person needs to accept clear and specific collection commands.
Enough amount of the
specimen has to be acquired to offer enough material for all required
diagnostic tests. The amount of specimen to acquire have to be specific inside
the lab p&p guide.
All specimens ought to
be positioned or accrued right into a sterile box to save you infection of the
specimen by using indigenous microflora and airborne microbes. Appropriate
sorts of collection devices and specimen boxes ought to be unique within the lab
p&p manual.
Specimens should be
protected from heat and cold and promptly brought to the laboratory so that the
consequences of the analyses will validly constitute the range and types of organism’s
gift at the time of collection. If transport to the laboratory is behind
schedule, a few sensitive pathogens would possibly die; therefore, certain
styles of specimens have to be rushed to the laboratory right away after
collection. Certain specimens must be positioned at the ice for the duration of
shipping to the laboratory, while different specimens ought to in no way be
refrigerated or positioned on ice because of the delicate and touchy nature of
the pathogens. Obligate anaerobes die when uncovered to air. Any indigenous
microflora in the specimen can also overgrow, inhibit, or kill pathogens.
Specimen delivery commands ought to be contained within the lab p&p manual.
Hazardous specimens
should be treated with even more care to keep away from contamination of the
courier, patients, and healthcare professionals. Such specimens must be
positioned in a sealed plastic bag for instant and careful delivery to the
laboratory.
On every occasion,
feasible, sterile, disposable specimen bins ought to be used. If reusable boxes
are used, they should be cleaned, sterilized, and nicely saved to keep away
from contamination of the specimen by microbes and doubtlessly harmful chemical
compounds.
The specimen container
needs to be properly classified and observed by using an appropriate request
slip containing adequate commands. At least, labels must contain the patient's
name, medical institution identity range, and room number; the requesting doctor's
call; the way of life site; and the date and time of collection. As a minimum, request
slips ought to contain the affected person's call, age, sex, and clinic
identity number; the name of the asking for the doctor; particular facts
approximately the kind of specimen and the web site from which it turned into
accumulated; the date and time of collection; the initials of the person that
accrued the specimen; and records about any antimicrobial retailers that the
patient is receiving. The laboratory must usually take delivery of enough scientific
records to resource in performing suitable analyses. As an example, the request
slip that accompanies a wound specimen should not simply country “wound”;
instead, it ought to nation the precise type of wound (e. G., burn wound,
canine bite wound, postsurgical wound infection).
specimens ought to be
amassed and added to the laboratory as early inside the day as viable to give
the technologists enough time to method the material, especially when the
sanatorium or health facility does not have a 24-hour laboratory service.
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